Goreisan alleviates cerebral edema: Possibility of its involvement in inhibiting aquaporin‐4 function

Aim In Japan, goreisan (GRS) is used to alleviate cerebral edema and relieve headaches. Although improvement of water maldistribution in the brain may be one mechanisms action GRS for headache, scientific evidence limited. Here, we aimed investigate mechanism against headaches, focusing on dynamics brain. Methods We a well-established mouse model evaluate effects related symptoms content. The was subjected magnetic resonance imaging (MRI) using H217O assess effect permeability through aquaporin-4 (AQP4) also examined vitro. Results inhibited loss righting reflex improved survival by ameliorating increase MRI evaluation suggested that influx into Evaluation via AQP4 Xenopus laevis oocytes HEK293 cells showed function. Conclusion suppressed ubiquity edema, suggesting inhibition function (not expression) as GRS. Our findings provide fundamental clinical efficacy